FastQCFastQC Report
Thu 30 Jun 2016
MG394676V_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394676V_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501099
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGCA700.00906570349.0859269
GGAGGGC1058.1838365E-443.6319358
AGAGGGC1551.2431928E-436.9463968
CACACAC1300.00233803435.2411773
TAGGGGG1400.003362210732.723955
GAGGGCA1802.9928444E-431.8149539
TAGGGTC1450.00399254931.5955415
CCTACAC3203.631394E-728.6334573
CTAGGCA1600.00646082528.6334574
CTACACA4103.6397978E-927.9350814
TAGACTG3007.117016E-626.7245585
GACCGTG2209.683522E-426.0304157
GTATATG5752.1100277E-1023.9027121
TACAGCG3851.8229348E-623.7992367
TACACTG3952.2782388E-623.1967245
TAGGACT3002.1832684E-422.9067654
CTACACC5806.0626917E-921.7219334
CTGTGCA2650.002860298121.6101579
ACCGTGT2700.003187877121.2099698
TAGTACT3854.9032442E-520.8243314