FastQCFastQC Report
Thu 30 Jun 2016
MG394658S_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394658S_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769701
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCC2401.3742138E-633.0095373
ACCGTGC3559.990035E-725.504358
TAGGACC2801.4839698E-424.2519054
AGGGCAG3351.838348E-523.6486235
TTATACT11300.022.0341784
CTATACT7001.2551027E-1021.0183184
GGACCGT3405.515846E-419.9721576
TATACTA7953.274181E-1119.9302865
TTAGGAC6251.6476406E-819.91893
AGGACCG2850.004674419.855365
ACATTAA10000.004668312619.85215235
CAGGACC2900.005169021419.5130254
TAAGACG3557.3764217E-419.1282634
TACACCG7153.7234713E-918.99455
GTATAAT15000.018.862591
CCTATAG3658.891115E-418.60422
TACCGTG3658.891115E-418.60427
CAGCCCC3050.006916892718.5533714
GTTATAC8058.1672624E-1018.2767983
TATACTT15250.017.8112355