FastQCFastQC Report
Thu 30 Jun 2016
MG394658S_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394658S_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769701
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAG2901.8252715E-423.5227431
TATACTC6808.0035534E-1121.7354765
GTTAGGG2650.002986629321.4515573
TAGGACT5306.0324965E-821.4515574
CTAGGAC3754.2408632E-521.222743
TAGGGGT2750.00370205920.6715014
GTCTTAG5601.0790973E-720.3023681
CAGGACT4609.10107E-619.7727414
GTATAGG5201.1910524E-619.677681
TTAGGGC2950.00555729319.2700423
TTATACA17050.018.6710344
ACTGGTC6703.4724508E-818.6660588
TCTATAC7455.858965E-918.3130073
ACTATAC6253.4277582E-718.1909223
GTACTAC3150.00811485418.0465491
CTATACA7851.1192242E-817.3798624
GGACTGC3950.00146484517.2698616
TATATAC9252.6375346E-1017.2076283
CAAGACC6656.5678614E-717.096734
TATATAG7351.00082616E-717.0153185