FastQCFastQC Report
Thu 30 Jun 2016
MG394536Y_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394536Y_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352431
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG1904.5283246E-429.6414995
AGACTGA2754.1148087E-628.6714126
GTCCACA1650.00814212727.3061081
CCTATAG1650.00814212727.3061081
CCCATGC1650.00814212727.3061088
ACTACTT6800.00927704826.56329235
CTCTAAG1700.00941809826.5029871
CTATACC2901.9414637E-423.3043523
ACTCTCC2500.002245501622.527546
GTATAGG2650.00314931321.2523981
ACTGCAC2750.003903454920.479588
CAGAGGA2850.004799929419.7609984
TCCAGAG4007.47342E-519.71162
TATACCT3506.913185E-419.3093194
ATACAGG4700.0049619319.215998235
AGCCCAT3000.006455996518.772956
CCTCCTA3000.006455996518.772959
TATAGAC3608.355335E-418.772953
TACTCTC3050.007102181718.4651975
TGTACAG3050.007102181718.4651972