FastQCFastQC Report
Thu 30 Jun 2016
MG394536Y_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394536Y_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352431
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTAG700.00943958648.583391
CTAGGAC1250.00202779136.2755973
GTAGTAC1650.00789170427.4815128
TATACAG4357.617928E-926.0600535
CCTACAT2200.001027674325.7639183
AGCACAG2901.8590252E-423.4540485
CGTATAC3002.3388983E-422.6722493
CTATACG2500.002163481222.6722494
AGGTGGT2500.002163481222.6722496
ACATAGC3052.6157318E-422.3005716
CAGTACT2550.002427283422.2276944
CTGTACA3153.253362E-421.5926154
TATAGAC3203.6185994E-421.255233
GTGGTCA3254.0179674E-420.9282289
TCTAGGA3405.447191E-420.0049272
CATATAC4609.310355E-619.7149983
TCCTACA2900.00511486119.545042
TAGACAG3557.2847924E-419.1596475
GTATGGT3608.0033013E-418.893546
GTATATA4201.0348623E-418.893541