FastQCFastQC Report
Thu 30 Jun 2016
MG394535H_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394535H_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548802
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTGG957.754088E-659.0955436
AGGGTGC700.009806192548.120656
CCCACAC1450.004400456330.974213
CCTATAC2901.8251012E-730.974213
TATAGGG2507.246947E-526.9475675
TAGAGGG3052.79342E-422.088175
TATATAC6851.03682396E-1021.30893
ACAGACC3203.8639695E-421.0527868
GCAGTTT10750.003860264320.508417235
TATACAG7853.092282E-1120.024735
TACGCTG3405.815672E-419.8143865
CTATACT5701.481676E-719.6985134
CTGTACG4650.004724488618.964771235
TACACTG4801.4621748E-518.7135895
GACCGCT3200.00954516317.543997
GTGTACC4501.8913395E-417.4660151
ACACTGA5904.4818626E-617.1276916
TACACCG5404.023376E-516.63435
CCCTTAT4752.8580468E-416.5467511
GTATACA4903.622104E-416.0402164