FastQCFastQC Report
Thu 30 Jun 2016
MG394535H_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394535H_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548802
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA1550.005932683629.1382037
TAGGGGA2855.338772E-627.7324125
GAGCCCC350.00797190927.431095175-179
GTCCTAT2701.17734264E-425.0912281
ATAGTCC3151.1613103E-525.0912286
CTTAGAC3151.1613103E-525.0912283
GTATATA7350.023.0429651
ACACTCC2952.1465035E-422.9648558
AGTACTG4704.814774E-721.6211665
TATAGGG2650.003107614621.3038733
GTTATAC5059.601172E-720.1226713
GACAGTC4007.345261E-519.7593427
TCCTATA4108.879645E-519.2774092
GTCTACG2950.00578153519.1373791
TATACAG7856.020855E-1018.6985595
GTCTTAG4301.2795813E-418.3807851
ATAGACC4351.3980847E-418.1695123
ATGTAGG4401.5259493E-417.963041
ATACAGG7006.1943865E-817.7430846
TAAGACA6556.0170714E-717.2382494