FastQCFastQC Report
Thu 30 Jun 2016
MG394206S_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394206S_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817415
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG1451.5651149E-647.170155
CAGCCCT2506.541058E-527.3586869
GTATAGG4306.373739E-926.5103551
TATACTG10450.025.0897055
GGGGGCT2450.0018644923.2641899
GTAGTGC2500.002096901222.7989048
GAGGGGG2600.0026334621.9220267
CCTATAC4855.948914E-721.1536243
GGGGAGT3304.2963933E-420.7262766
GTTAAGG2800.004047009620.3561651
TCCTATA7302.0008883E-1020.3003942
TATAGGA7452.6375346E-1019.891662
TATACAC7452.6375346E-1019.891663
TAAGAGT5201.1613956E-619.7298224
ATAGCGC4851.4045589E-518.803228
AGAGGGG3050.006635326418.6876286
TAAGGTG4351.3003916E-418.3439465
CTATACC5051.989118E-518.0585384
AGTACCG4451.5476414E-417.9317235
GAATAGG6404.2766987E-717.8116461