FastQCFastQC Report
Thu 30 Jun 2016
MG394206S_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394206S_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817415
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG3501.8189894E-1135.902531
TAGGCCC1600.0065460828.5588284
CTAGAGC3355.5525743E-727.2800753
TATAGGG3559.211399E-725.743175
CAGGGTA3405.1804015E-420.1591749
TAGAGCT5751.3565113E-719.867014
TAGACAG7002.5484042E-919.5831995
CTCTATG3100.00719984318.4250532
GTGCTAT4351.2795345E-418.3826941
CAGTCTA3750.001001483218.277659
TATATAG6904.6064088E-818.2114282
CAACGCG3150.0078957918.1325919
TGTGTGC3200.0086459317.8492688
CCGTACC3850.001194924717.8029069
TAGGCAC3250.0094534817.5746655
TACACAG7308.771349E-817.2135415
CCGTGCA4652.1314439E-417.1967149
TACACTG8051.4406396E-817.0288685
TCTAGGA8101.555054E-816.923752
TATACAG11701.8189894E-1216.5982935