FastQCFastQC Report
Thu 30 Jun 2016
MG394203X_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394203X_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697531
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTC650.007119124352.1834568
ACCGACC2102.1044658E-532.3040478
ACCGTGT3952.5511708E-622.899078
TGCACCG4452.8090471E-722.8669095
CCGTGTA2500.002198871522.6128319
GCACCGT3052.6662115E-422.242136
CACCGTG3102.9761466E-421.8833857
CTAGGAC3102.9761466E-421.8833853
CTAAGGG2650.003084032821.3328593
TCTAGGC3203.6882987E-421.199532
CCGGTCA2850.004700672319.8358179
TAAGGGT2900.00519805519.493824
GTATAGG4159.64919E-519.0710621
CTATACC4751.2591232E-519.0423834
GCAGTGC3608.1566843E-418.8440278
ACACTGC3608.1566843E-418.8440278
TACACTG6603.1133823E-818.8440255
TATACCG4251.15829556E-418.6223345
GTCTAGG3150.00837777517.9466931
CGTATAG4451.6473774E-417.7853741