FastQCFastQC Report
Thu 30 Jun 2016
MG394203X_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394203X_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697531
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTG1650.00790959227.472481
AGTGGCC1700.00914931526.6644658
CCGTGTA3007.734592E-626.442269
TATACAG10850.026.1115155
AGGGTGA2801.4706404E-424.2837096
TAGCCTG2801.4706404E-424.2837095
CCTATAC4802.1847882E-823.6091613
TAGACAG6206.202754E-1021.9336725
TATACCG4704.6520836E-721.7003365
TATACTG9000.021.405645
GTACTAG4355.7725556E-620.8411921
TATATAG6551.2314558E-920.7616465
GGGGGGG600.003638437520.73587205-209
CCTAGGA2800.004187031620.2364232
CAGTGCG2800.004187031620.2364239
GTCTAAG3956.486536E-520.082731
GGCTGTC3405.4666423E-419.9983487
TACTAGC7953.092282E-1119.9564252
TACACCG6351.9470463E-819.6309265
TATATAC9751.8189894E-1218.5967563