FastQCFastQC Report
Thu 30 Jun 2016
MG393070J_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG393070J_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637975
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGGC1250.00195210136.562338
CTAGGGT1550.005599378629.4857463
GTGTACG2659.568935E-525.869571
TACTGCG3151.0596488E-525.3905037
GTCCCAG2400.00163129723.8035981
GTAGACT3851.8630035E-623.741774
TAGACTG3502.3957564E-522.8514545
TATACTG7451.0913936E-1121.4711635
TTATACG7601.4551915E-1121.0473924
GCACTGT2850.004423250420.0451356
ATACTGT9150.019.9794146
GTATACA6301.6174454E-819.9496821
GTGTGCA2900.00489147319.699531
GTACTAG3506.287406E-419.586961
GTCTTAG4701.04952105E-519.4480461
TAGGGTA2950.00539961719.3656394
CTTAGTC3556.917334E-419.3110893
CGTAGAC3000.00595028719.042883
CTTATAG4251.0685195E-418.8188443
GTCTAGA3658.3383673E-418.7820191