FastQCFastQC Report
Thu 30 Jun 2016
MG393070J_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG393070J_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637975
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG650.00675374352.882879
TATAGGG2703.1275758E-629.705813
TAGGACC2506.3148465E-527.4990924
AGTGGTC4407.70342E-926.0408088
CTAGACC3701.2862929E-624.7739584
TGTGTGC2801.364512E-424.552768
CTGTGCA3751.4457946E-624.4436389
TAGACTG3401.8733985E-523.5899075
CTATAGG2850.004351728620.1016752
GAATAGG5151.0071944E-620.0236111
GTACTGT5851.5746446E-719.5862486
GTATAGC6552.4481778E-819.2423691
CATACTG5451.732662E-618.9213945
GTGGTCA5501.8908631E-618.7493829
TAATACT12150.017.9177914
GCACTGT3250.00928894417.6276236
TATACTG8551.546141E-917.4214535
GTTCACG5302.8817965E-517.2950271
GTCCAAG6656.0510683E-717.230011
ACATCGC5403.383219E-516.9747498