FastQCFastQC Report
Thu 30 Jun 2016
MG392174G_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG392174G_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717165
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG7200.028.3382845
GTAAGAC4452.7409624E-722.9253543
CACCGTG3002.3433121E-422.6706287
CCTATAC4906.9286943E-720.8199623
GTACAGG6001.01154E-820.7814081
CTATACT7101.4915713E-1020.75484
TAGAGAG3855.3146545E-520.6096615
TCTACAC7752.1827873E-1120.4766963
ACACTGT5701.3419776E-719.8865156
TAGACTG4007.131541E-519.8367985
CTTAGAG4057.8460056E-519.59193
TACTCTG7053.0649971E-919.2941515
CTTACAC5301.4708748E-619.2486443
CTGTGTG2950.00565397219.2123979
CTAGACT4251.1359017E-418.6699284
GTAGAGT3658.797143E-418.6333926
ACCGTGC3100.0075282118.2827638
GTATGGG5001.916207E-518.1365011
GTATATG9401.8189894E-1118.0882661
ACCGTGT4451.615591E-417.8308328