FastQCFastQC Report
Thu 30 Jun 2016
MG392174G_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG392174G_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717165
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG5601.6734703E-1024.3534745
CTATAGG2952.0545769E-423.1151623
CCCTACA3052.5747632E-422.357292
ACACTGT6104.8748916E-1022.357296
TATACCG5559.854193E-820.4773965
CACTGTG3354.8525038E-420.3551437
GTGTAAG5251.3100162E-619.482781
TAGTACT6703.486639E-818.658884
GTATAGG4901.5744907E-518.5550291
GTATTAG14250.018.3434351
TCCGTGC3100.00741567318.3305728
TAACACC6854.4930857E-818.2502924
GCACCGT3150.00813232818.0396126
CTTAGAG3150.00813232818.0396123
TATAGGC3800.001132831417.9446642
CTAATAC13350.017.8774593
GTGTATG7106.769733E-817.6076771
CTATAAC6505.191687E-717.4845473
TTAAGGT3900.001348360917.4845474
TGCACTG3900.001348360917.4845475