FastQCFastQC Report
Thu 30 Jun 2016
MG391826E_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391826E_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674723
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCCC1450.00409881831.4279869
GTCTATG4655.002221E-1026.9503441
TATAGTG6054.274625E-1022.5969835
GTATAGG4553.2340904E-722.5349031
ACACTGC3552.7341219E-522.4643696
TATAGCG6601.2660166E-920.71395
GTATAAT13950.020.4169251
CCGGTCA2900.0049744819.6424929
GTATAAG6452.1917003E-819.4293171
GTACTAG3557.0542045E-419.2551751
AGGGAGA3658.503168E-418.7276346
TATACTA6703.3898687E-818.7043425
ATATAGG4301.19519595E-418.5461656
TGTACAC3709.317158E-418.4745583
GTCTAGA3800.001114392917.9883861
AGTGGTC5102.1752632E-517.8708158
GTCTAAG3200.00878029817.8010061
GCACCGA4501.6929657E-417.7218936
GTATAGT5803.3172928E-617.6782421
GTGTTAT7801.0077201E-817.5271451