FastQCFastQC Report
Thu 30 Jun 2016
MG391826E_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391826E_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674723
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTG3102.835459E-729.4572895
GTGTAGG4503.3287506E-1027.9025961
TAGGGCT1700.00883192226.8581164
TAAGGGC2350.001451320624.2865944
TTAGACT4653.914065E-722.0929664
TAAGGGT2600.002612636721.9513444
TCTAGGG2750.003617147420.7539982
AGGTCGA2750.003617147420.7539986
TACACTG6651.3587851E-920.5979525
TAGACCG3956.134943E-520.228585
ATAGTGG4557.998469E-620.0698018
GTACTAC2850.004448299320.025791
GTAGACC3000.00598391619.02454
TAGACTG3607.64996E-419.0244985
TAGGCAC3607.64996E-419.0244985
TACACAG5401.6451359E-619.0244985
TTATACA15100.018.8985084
GTTAGAC3658.393351E-418.763893
GTATAAG8107.912604E-1018.3198871
CCGTGTA3750.001006449818.263529