FastQCFastQC Report
Thu 30 Jun 2016
MG391787L_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391787L_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583638
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAG3402.0057618E-523.3820763
CCTACTC3002.3124588E-422.7140163
ACTGTCC4603.701607E-722.2202328
TATACCG3653.4699926E-521.7805655
TATAGTG5301.4432426E-619.2854845
CTCCCAG3050.006777461618.6180461
CTACTAG4901.5831823E-518.5420531
GTTACAC3709.513203E-418.416773
GAGCGTA3100.00744403318.3177577
GCGTACG3100.00744403318.3177571
TATACTG6253.4634286E-718.1712135
TGCAGAG3750.001041044718.1712132
TTATAGG3900.001354286817.472324
GTGTACA3900.001354286817.472321
GTATAGA5853.707728E-617.472321
TAGACAG4652.2273159E-417.0965715
ACTGAGC4000.001604540717.0355138
GGATAAG6757.757517E-716.8251971
GTATTAT11501.8189894E-1216.788621
AGAGCGT4752.6201512E-416.7366436