FastQCFastQC Report
Thu 30 Jun 2016
MG391787L_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391787L_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583638
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCACA2550.001232067540.14364235
CCCTTAG1755.726026E-639.0601581
ACAGGGC1806.9475427E-637.9751558
CTCTAGG3600.037.9751551
ATAGGGC1400.003451537632.5501336
GTGTATG4701.8189894E-1129.0873551
AGTACCC1600.00663195728.4813634
TCTATAC5353.6379788E-1227.6828233
ACAGTGC2659.758586E-525.7944458
CTAGTGT3851.9102627E-623.6728233
TAGACCG2901.799478E-423.5707865
GTCCTTA2901.799478E-423.5707861
CTCCAGG3452.1918839E-523.115311
TAGACAG4603.5918674E-722.2897625
TATACTC4355.511447E-620.9518095
GTACCGC4006.857871E-519.9369566
GGACTGC2900.00497394119.6423236
ACTACTG5801.527369E-719.6423235
GCACCGA4159.099872E-519.2163436
GTATATA6552.613342E-819.132521