FastQCFastQC Report
Thu 30 Jun 2016
MG391785X_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391785X_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790586
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGG1150.001345961139.4381147
CTGACGG4100.002096776636.02469235
TCAGGGC1450.00419601431.2785053
CAGTGCG4204.241014E-621.5970619
CCTATAC4957.6202195E-720.6153773
GTATAAG7553.3833203E-1019.523171
GTACAGG7002.7976057E-919.4373551
ACCGTGC2950.0056454219.2177268
ACAGTGC4201.0352989E-418.8974298
CCTAGTG4901.6068065E-518.5117682
TATACTG10450.018.4453375
GTATACG6905.0171366E-818.0758021
GTATATA10800.017.8475721
GTACTAG4501.7563342E-417.63761
GTATATG9001.8553692E-1017.63761
CATAGAG3250.00986887917.443785
GGACCAT5202.6787735E-517.4437796
TCTAGAG5303.154701E-517.1146532
TTAGTCC4903.3645576E-416.1977964
CTAACAC5655.4564138E-516.0544533