FastQCFastQC Report
Thu 30 Jun 2016
MG391785X_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391785X_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790586
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAC9950.024.0210763
TATACTG9550.021.4518815
CATAGAG2750.003679423120.693483
TAGACCG3354.8059743E-420.3846235
CCAGGAC2800.00408417420.3239543
CTAATAC15700.020.2980653
TGTAAGG4558.205825E-620.0112782
TAAGGGT3506.4559374E-419.5109984
GCTGCGC3658.561493E-418.7091758
ACAGCGC3050.00669606218.6580568
GTGTAAG6102.6264752E-718.6580561
CCTACAC5552.199602E-618.4563483
TAGGTAG3100.00735468218.357125
TACACGG3750.001026588318.2102645
ACACGGT4401.4366159E-418.1067966
CCTTAAC3150.00806548418.0657393
TTAGACT5702.8378054E-617.9706554
GTATTAG14050.017.8214321
TAAGGTG3850.001224844517.7372685
CCGTACA5152.3865614E-517.6798679