FastQCFastQC Report
Thu 30 Jun 2016
MG391778Z_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391778Z_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701421
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2107.807394E-427.0102484
AGTGTCC2700.003371072721.007978
CCCTATA3658.7501376E-418.6481721
GTCTAGC3658.7501376E-418.6481721
TATAGCG6102.7171154E-718.597225
CCTTAGA3750.001049182318.1508871
CCCTTAA3800.001146703717.9120581
ACGGTGC3200.0089983517.7254758
CTACGTT4702.438476E-416.8957734
TATATAC12150.016.8063773
GTATATA7451.1967859E-716.7499791
ACAGAGC4100.001907472616.6014218
TGCACTG4100.001907472616.6014215
GTACCGA6206.5197546E-616.4675396
CTATAGA6307.5902626E-616.206151
GCTTAGA4953.6200596E-416.0424521
TATACAG9957.6579454E-1015.9618345
CCTAGAA6501.0210802E-515.7074972
TCTATAC7952.5058762E-715.6965213
GTTCTAG5806.794466E-515.6473161