FastQCFastQC Report
Thu 30 Jun 2016
MG391778Z_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391778Z_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701421
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG4858.1854523E-1025.830485
GTGTATG5252.050001E-923.8624421
TGTAGGG4301.8809988E-723.837222
GTGTAGG5453.1595846E-922.9867571
CCGTGTG2500.002107930422.7777869
TGTGTGC2500.002107930422.7777868
GTATAGG4103.3103497E-622.2222331
ACAGAGC3153.157139E-421.693138
CTAGAGT3203.5116298E-421.3541763
TATACCA11300.021.1652015
GTGTATA6501.0513759E-921.025651
TACCGTG3253.899254E-421.025657
CTATACC5559.637006E-820.5205294
GTATAGA7402.4374458E-1020.0075151
CATACAC5901.8361425E-719.303213
CGTAGAC3658.5224665E-418.721473
CACTGAG3150.00803412318.0776087
TAAGCCC3150.00803412318.0776085
AGTACTG5702.8191444E-617.9824625
GCATAAG8401.3242243E-917.6256681