FastQCFastQC Report
Thu 30 Jun 2016
MG391777G_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391777G_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688238
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGG1200.001597113838.0879971
CAGGGCA2209.82148E-425.9690889
ACAGGGC2400.001630868923.8049988
TACTCCG3052.4823734E-422.478165
CTGTGCG2650.00290080421.5592449
TAGTACC3754.0794752E-521.3292774
GTGTAGG4355.3739714E-621.0140671
TAGACAG6151.2256351E-820.4374625
ACCTGTG3956.087003E-520.2493137
ACACTGG3405.170508E-420.1642326
CCTGTGC2850.004422094720.0463128
TGTATAC6552.5278496E-819.1893713
TAAGGGG3000.00594873419.0439974
TATAGGC3050.006544485718.73182
ACAGTGC4351.2767043E-418.3873088
TACACCG6954.9853043E-818.0849485
CCTATAC3900.001300433317.5790733
TATACAG9202.2737368E-1017.3879995
GTCTAAG3950.001416320317.3565541
CAGGACT5353.1990865E-517.0862054