FastQCFastQC Report
Thu 30 Jun 2016
MG391777G_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391777G_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688238
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG1200.001573327338.204515
GACTGGG3650.00704240528.129051235
GTCCTAG2557.212478E-526.967891
CCGCGTT3151.0345235E-525.4696719
AGTGTGT4059.9129466E-825.4696718
ACCGCGT2400.001602129723.8778178
AGGGTGA2450.001806382323.3905146
TATACCG4103.1329364E-622.3636135
TAGTACC4203.8613907E-621.8311464
GTGTAGC6959.640644E-1121.43851
CAGGACC2850.00434461120.1076354
CTTACAC4659.317409E-619.7184543
GTATAGA5251.2080109E-619.6480331
TCAGGAC5401.5822334E-619.1022553
GTAGTGC3000.005844698819.1022538
TATATAG6202.8935938E-718.4860525
GCACAGT3150.007745545418.1926211
TAGAGTC3200.00848151417.9083639
CTGTAGT6404.0382474E-717.9083634
TAGGCGT3900.001274077917.632855