FastQCFastQC Report
Thu 30 Jun 2016
MG391770J_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391770J_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171355
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGGG1700.0091865726.6437369
CTAGGAC4558.5808515E-619.9096073
TATAGAC9009.094947E-1218.8726482
CCTATAG6052.543893E-718.7166751
ACCTCCC3100.00757606518.2638518
CTAGGGG3100.00757606518.2638514
CTATAGA9951.8189894E-1218.2087841
GTATAGT8851.4915713E-1017.9130211
GTCTAGG5202.71094E-517.4209061
CCTATAC6505.401089E-717.4209043
CAGGGCA3950.001505562817.2003889
GTATATA13900.017.107581
ATTATAC19200.017.1033363
GTCCCTA4050.001779779416.7756861
TATACCG7101.3591743E-615.9487155
TAGACTG6601.2020391E-515.4412575
AGTACTG8955.9244485E-815.1824655
CTAGACT5255.745549E-415.0981184
ATATAGG7502.4049768E-615.0981181
TATAGAG10501.6880222E-915.0981182