FastQCFastQC Report
Thu 30 Jun 2016
MG391770J_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391770J_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171355
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG13250.021.4178735
GTATAGA11900.020.9859141
GGACTGC7352.3283064E-1020.0774356
TAACCCG4558.400431E-619.9586325
CTATACG7603.6561687E-1019.4169944
GTATAAT19400.019.3092061
GGAGTGC3557.232499E-419.1855878
CCTACAC7805.16593E-1018.919123
CTAGGAC4801.332902E-518.919123
ACTATAC9101.0913936E-1118.7112183
GTACAGG10600.018.2051931
CATATAC13750.018.1623573
GTAAGAC6253.4886216E-718.1623553
GTATATA13550.017.592691
CTGAGTG3900.001359826117.4638049
GTAGTAC4752.6327564E-416.7284853
CCTTCCG6156.0074144E-616.6119129
TACACAC6206.4881715E-616.4779455
CAGTGCC4200.002232478216.216399
GTCTAAT10509.458745E-1116.2163891