FastQCFastQC Report
Thu 30 Jun 2016
MG391498T_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391498T_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957217
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAG3005.075507E-448.06782235
CCTTAGG4100.001977620226.378685235
GTATATA11500.024.529971
GGGCGCT2450.001978665823.0281339
TATACTG13100.021.5339415
GTCCTAG4305.424299E-620.993091
CTAGGAC4406.61838E-620.5159743
CTATACT9600.019.9817054
TAGGACT6251.7065759E-819.8594654
ATCTAGG6301.870103E-819.7018471
CCGTGCA4251.1768068E-418.585069
GAGAGTG6253.7128848E-718.0540587
TATACCG6905.3058102E-817.9886465
ACCGTGC3200.00928225317.6309158
TATATAG8602.053639E-917.0568872
TGGACCG4652.3420704E-416.9863435
GTATAAG10459.640644E-1116.1968231
GTATAAA21400.015.8183921
TTATACG12153.6379788E-1215.7880134
CTATGGG6459.991203E-615.7448181