FastQCFastQC Report
Thu 30 Jun 2016
MG391498T_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391498T_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957217
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC1550.00586177829.2121878
ATAGGAC4101.2600503E-724.848173
CCTAAGG2851.6714938E-423.8309943
GTATATA12950.023.6009651
GTGTAAG7700.022.0514071
CCTAGAC4654.2458305E-721.9091383
CTATACT10700.021.158364
GTACCGT6601.373337E-920.581316
CTTAGTG5651.2418968E-720.0349042
TCCACCC3405.5100827E-419.9759798
ACCGTGC4651.0380338E-519.4747898
CCTAGCC3557.368729E-419.1319249
CCCTATC3608.0954516E-418.8662038
GTACTAA10450.018.4148581
ACAGACC6203.300629E-718.2576168
CCACCCT3100.00759001118.2576169
ATACCGT11900.018.0735056
ACTGAGC5052.113524E-517.9322328
CCTATCG5702.9902585E-617.8732459
GTACTAT6404.605372E-717.6870651