FastQCFastQC Report
Thu 30 Jun 2016
MG390984V_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390984V_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802724
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT8550.029.10154
TCCTAGG3002.3795283E-422.61982
AGGACCT3102.970492E-421.8901315
TATAGTG8251.8189894E-1220.5634565
GGGGGGG600.00428934420.155190-194
GTATATA10150.020.0569671
TTAAGGT3456.1139726E-419.6693944
CCTATAC5251.3728204E-619.3884013
GTGTAGA7204.094545E-918.8498341
AGGTCTG3050.00694420118.5408237
CTAGCAC4351.3813467E-418.199843
CTAAGGC3150.0083639817.9522233
ATATGTG6600.00325390217.852354235
TAGAGGG4451.6438875E-417.7908555
AGTGGTC5753.2758344E-617.7024528
TGTAAGC6758.1114376E-716.755412
ATAGTAC6105.7514953E-616.686743
AGGCTGC5454.0954037E-516.601696
TTATACC9705.5842975E-1016.3235684
AGTACAG10601.1459633E-1016.0045785