FastQCFastQC Report
Thu 30 Jun 2016
MG390984V_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390984V_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802724
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCCC700.00936958848.68078
ACCGGGC1500.00490969230.290218
AGTGTCC3755.1322786E-827.2611928
CCTAGGA2608.751736E-526.2126832
AGGGGCG2650.003002785421.4317537
CCCTATA3203.575514E-421.2978082
CTAGACC3754.2714546E-521.203154
TCCTAGG2950.00558723819.2522531
ACCGTCC3658.676706E-418.6720498
ACGGTGC3050.006773354518.621038
GATGAGG6850.00720281818.420368235
GGGCTGG3100.00743952118.3206946
TACGGGG3150.00815846218.029897
TCTATAC8851.4188117E-1017.968773
CCTTGGG3850.001241290217.702072
TATACTC7157.383278E-817.4751225
ACTGTCC5954.355181E-617.1814238
GTATACC5303.1065705E-517.1454031
GTCTATA6707.173312E-716.9534761
ACACTAC4050.001742522116.8278963