FastQCFastQC Report
Thu 30 Jun 2016
MG390982M_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390982M_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586156
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGA550.003661756961.7275356
CAGTGGG1650.00796219927.434469
TAGTACT4256.0736056E-926.6275654
TGAGCCG2651.02118196E-425.6227515
GTATATG5851.0913936E-1125.1482561
TACACTG4951.1150405E-925.1482545
ACTGGGG2300.001346160524.6015553
CTTACAC4251.785429E-723.964813
TAGAGGT2450.001944524723.0953373
ATAGGAC3354.992198E-420.2687443
CTCCTAG2850.00467510319.8538861
GCCCGGG600.004814581519.756405170-174
TATACCG2900.005169797719.5115785
ACCGTGA3506.7056686E-419.4000848
ACTATCC5602.528317E-618.1875788
AGTACTG4401.4998784E-418.0038645
TATGCCG3900.001386936417.4103325
GTACTGC3950.001510477617.1899476
GTCGTAG3950.001510477617.1899471
TGTACTG6606.3644984E-717.1465385