FastQCFastQC Report
Thu 30 Jun 2016
MG390982M_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390982M_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586156
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCACC1450.004140428331.3625144
ACCGGTG1700.00900577926.750388
GTCCTAG2200.001011304125.8384341
GTCTATA3651.2225919E-624.9181611
TATAGGG2450.001893175423.201867
GCAGTGT3002.2961346E-422.7378228
GCTATAC5553.974492E-922.5329783
CCGTCTA3653.442093E-521.803399
GTATAGA5806.6083885E-921.5617281
CTATACC3754.239836E-521.2219684
CTATAGA3804.6954512E-520.9427321
CCGTAGA2800.004109001320.3016282
GTAGACC2900.005034059319.6015724
CTATACT6602.9202056E-818.9481854
TGCTATA6002.2306813E-718.9481852
TACACTA4251.1099703E-418.7252675
TCTAGAC3050.00673664618.637562
GTATAAT11000.018.6036741
GTGTATG6203.1485433E-718.3369541
CCTATAC3750.001033757118.1902583