FastQCFastQC Report
Thu 30 Jun 2016
MG390975X_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390975X_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446688
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG1150.001379599739.236839
CACACTA1904.4908316E-429.685769
GTGCTAC2200.001057987225.6377011
GTCTTAG2701.1842968E-425.0679761
TACACTG3653.6530466E-521.6340055
GTATAGC4558.847441E-619.8340021
GTATTAC4701.17063955E-519.2010021
TATAGCA5301.5379846E-619.1557182
ACACACT2950.005810931319.1196428
GGTATAC2950.005810931319.1196421
CTATAGT3000.00640324418.8009824
ACTATAC3709.949476E-418.2928473
ACCTTGC3709.949476E-418.2928478
CTAGTAC3200.00928987617.625923
AAGTGGG3200.00928987617.625926
GTACAGG3850.001298937517.5801371
GTATATG5803.6396505E-617.504363
ACTGTTC7901.3293175E-817.135078
TAGATCT3950.001542386717.135074
CTAACGT4000.001677871816.9208834