FastQCFastQC Report
Thu 30 Jun 2016
MG390975X_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390975X_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446688
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATC2056.766916E-427.6767588
CAGACCG2107.790222E-427.0177849
GTCTATA2956.711165E-626.92622
GAGAGTC1700.00908678226.699935
CTAGACC2557.713678E-526.6999284
TGTAGAG2557.713678E-526.6999285
CTAAGAC3007.6471115E-626.477433
GCCTTAT4306.673872E-926.3894651
ACAGACC2158.9378114E-426.3894658
ATACTGT7400.024.535076
TTATACT6051.6370905E-1124.3829944
TCTAAGC2901.8475406E-423.4775242
GTACGAC3002.3244636E-422.694943
CCTTATA5604.498361E-922.2896752
CTCTACA2550.002414222822.249942
CTTATAC4103.4120803E-622.1414073
TCTATAC4204.205125E-621.6142273
TAGACAG4355.6989484E-620.868915
ATAGAGT3354.899165E-420.3238286
ACTACAC3354.899165E-420.3238283