FastQCFastQC Report
Thu 30 Jun 2016
MG386159C_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG386159C_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659529
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG4758.440111E-1025.7608411
AGGGTGC2700.003776559420.5999986
GTGTAGA3355.605928E-419.9235821
GGTGTCG3558.28197E-418.8011268
GTATAGT6103.3379E-718.2360651
AGGTGAG6754.850699E-818.1279987
GTACTAT5552.7355218E-618.0389181
TATATAG5052.453547E-517.6221775
GGTGAGC3800.001307749817.5642098
GTACTAA6405.5225246E-717.3812481
CGGTGCA3850.001427466217.3361039
GTATATC4853.597725E-416.0552561
CTAATAC9757.585186E-1015.9729223
AGTATGC5004.5360505E-415.5735998
CTATATA5054.89242E-415.4194041
TACCAGG4350.003226884215.3434482
CAACGCA4500.004043464514.8319999
TATAGGA6151.3231375E-414.4702432
TAATACC14650.014.427034
ATACTAC4800.00620544613.9051