FastQCFastQC Report
Thu 30 Jun 2016
MG386159C_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG386159C_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659529
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA2456.554571E-527.3492371
TATATAC3509.912947E-725.5259513
CTATACT7150.023.4285414
TATACTG7209.094947E-1221.7147875
CAGTGTG3204.0088085E-420.9392599
TAGTACA3906.5818116E-520.0444164
ATATACA2850.00504852519.5922874
CTTAGGC3507.333007E-419.1444643
GTATAGA4751.4004325E-518.8085981
GGGAGTC3100.00820306718.0122647
CTAGGAT4451.8104575E-417.5670193
GTCCTAT3200.00984897917.4493831
CTCGATG3200.00984897917.4493839
ACAGTGT5253.3116048E-517.0173028
TACACTG3950.001651024716.963455
TATACTA9353.947207E-1016.7215465
ACTGTCC4050.001951530616.54468
TATATAG4803.2270348E-416.286095
GTATAGG4150.00229690816.1459351
TGTATAG4853.492117E-416.1181932