FastQCFastQC Report
Thu 30 Jun 2016
MG291789G_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG291789G_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719484
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC2801.5180979E-424.170443
CCTACAC4953.18214E-822.786953
TATAGGA5407.9906386E-820.8880372
GTATACA7051.4551915E-1020.799151
GTCTACA6001.0706572E-820.6791571
TAGACTG4406.636188E-620.5082555
ATAGGAC3355.105731E-420.202163
GTATATG9550.020.0787621
AGTACGG3405.6418293E-419.9050715
GTAGTGT4058.1490965E-519.4955026
CAAGGGT3506.857901E-419.3363534
CTACACC9350.019.3018864
GTCATAG3608.288576E-418.7992321
GTATAGG4201.077343E-418.7992321
TATAGGG3659.093614E-418.541712
GTGCAGG4951.8336552E-518.2295591
CATGTGC3100.007745424818.1928064
ACAAGGC4351.4098239E-418.1509848
GTCTTAT7006.2751496E-817.724991
GTACTAC3200.00930021417.6242796