FastQCFastQC Report
Thu 30 Jun 2016
MG291789G_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG291789G_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719484
Sequences flagged as poor quality0
Sequence length50-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCGC1051.3297053E-553.9561658
ATAGGGG2253.3227087E-530.215453
GTACTGC4101.2472992E-724.8724776
CAGGGGT2350.001515133324.1080744
TATAGGG3801.7884558E-623.8543032
TATACTC6202.3646862E-1123.758125
CCTATAC4402.4674046E-723.1766243
TATACCG5403.0140654E-923.0812475
ACCGTAC3153.2682891E-421.5824668
CTATACT7601.6370905E-1120.8725154
CAGCTCG3804.8204733E-520.8725159
CAGTGTG4457.03612E-620.3699689
AGTGTGC2800.00419058920.233568
TATATAG7352.382876E-1020.0408615
TGGGGTG3405.472086E-419.995525
TAAGGGC3405.472086E-419.995524
GTATATA10300.019.8013881
TATATAC10400.019.610993
ACAGACC4057.870126E-519.584098
TCCTATA6052.5235568E-718.7285842