FastQCFastQC Report
Thu 30 Jun 2016
JG269965G_R2_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJG269965G_R2_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370370
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGC1551.5156087E-435.7196128
CGTCGCA1551.5156087E-435.7196129
GACTGTG1400.00396545231.637377
CCTAGTC2601.03918996E-425.553263
TATAGGC2701.3427982E-424.6068442
ATACTCC2751.5208457E-424.1594456
CCGTCTG2650.003477351720.8926039
CTTAGAG3406.381311E-419.5407283
GTATAAG4059.375534E-519.1386551
CTAGAAC3558.531704E-418.7150653
CTATACT4201.2392196E-418.4551334
CCCCCCC750.00837051317.95104220-224
CGATCGA650.00914085217.678988130-134
TATACTC3900.001603047617.0355075
ATTATAC6001.112967E-414.7641063
TTATATA4700.00555778414.1358462
GTCCAAA5600.001106999813.841351
ATTAGAC4850.006843802513.6986553
TAGATAG5800.001442590313.3640615
GTCTTAA6100.002108502712.7068121