FastQCFastQC Report
Thu 30 Jun 2016
JG269965G_R1_tr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJG269965G_R1_tr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370370
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC500.00265970566.909488
ACCGGTG1550.0063014828.778277
GTATAAG3902.8321665E-928.5937941
GGGCTGC1600.007358136627.878956
TATATAG2456.6112625E-527.3099925
GCTTAAG2701.2799246E-424.781291
CAGTACA2400.001877420223.2324589
AATACAG5155.4438715E-821.6535515
TTTATAC5408.9841706E-820.6510753
TAAAGTG5651.4480975E-719.7373095
TTATACC5701.5889418E-719.5641734
CTAAGGG3507.395803E-419.1169953
TATCCTG4201.1738962E-418.5859665
TAGACTG3050.007525038418.2812775
CCATAGG3750.001175590217.8425251
CATGGGT3150.00906263317.7009184
AAGACAG9052.5283953E-1017.2510625
GACAGTG3900.001528999317.1562777
TATGCTG4552.1660927E-417.1562775
TCTAAGC4552.1660927E-417.1562773