FastQCFastQC Report
Thu 23 Jun 2016
Undetermined_S0_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482623
Sequences flagged as poor quality0
Sequence length32-241
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC19550.40507808372166265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTG1551.1677039E-437.341435
GTATAGG1802.8082498E-432.161791
GTCCTTA1450.00378601531.9399811
CCTCATG1450.00378601531.9399811
ACTGGGC1450.003789859131.9333638
TGACCTG1954.4950025E-429.6816525
ACTGTCC2455.129644E-528.3490078
TCTAGTG2851.4346943E-424.3701972
GGTGATC2400.001511390724.1163448
TAGTAGT2600.00240739322.2612384
AGACTAC3152.8262718E-422.0492278
TACACGA2950.0050054119.6200755
GTGCTGG4208.807578E-519.2970731
AGATAGG3000.00551614619.2930746
TCTAGAG3000.00551614619.2930742
CTTATCC3657.634067E-419.0287863
CTGAGAT3150.007310954418.3743559
ACTATAC3250.00875413517.808993
AATACTC4752.2638949E-417.059147
GTAATAT4802.4484517E-416.884941