FastQCFastQC Report
Thu 23 Jun 2016
Undetermined_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482623
Sequences flagged as poor quality0
Sequence length32-241
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGGG802.0348074E-457.851567
CCCTATA1601.4114991E-436.1572232
GCACAGG1450.003798739831.9181026
TAAGGGC1550.005264309229.8588713
CTATACT3552.5152985E-829.3331854
GTATTAG5700.028.418311
GTATATA3057.4773707E-626.5548151
CCTATAC1750.0095201126.4464263
CGTATAC2209.125612E-426.2961623
GTATAGG2300.001182738625.1528531
ATAAGGG3251.2239678E-524.9206713
GTGTAGA3801.4891029E-624.3585511
TGTACCG3351.5478849E-524.1767715
TAAGACT3401.7360015E-523.821234
CTAAGAC2450.001708886423.6128833
AGCGGTG2650.00269641721.8307765
CCTATAT2700.003005338621.4265043
GGTTAAG2800.00371071520.661271
TCTATAC2850.004111288620.2987923
ATTACAC3455.240483E-420.1222823