FastQCFastQC Report
Thu 23 Jun 2016
MU585039B_S16_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMU585039B_S16_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606634
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTC3753.6379788E-1133.669047
ACAGGGC2303.53387E-529.9428238
CCCTAAC2005.474766E-428.6999361
TAAGGGC2056.331581E-427.9953234
GCGTCGA1700.00859594227.007259
CTTGGCC2400.001588366923.912679
CCTATAT3952.2340682E-623.2505821
GTATAAA10500.022.959951
CGTGTAC2500.002014161622.9561633
CTACACT4052.7800252E-622.6727524
TACACCG5603.949026E-922.546235
GTCTATA4103.0880929E-622.399951
GTCTAAG3152.9909864E-421.866621
TATACAG9500.021.7479445
GTATAGG3203.3269214E-421.5249541
TTACACC6601.1532393E-920.869244
CTACGCT2750.003502516320.8692384
GTATATG7251.6552804E-1020.5847821
ACACCCA2800.003887931120.4965725
ACCGTCT5609.7510565E-820.4965728