FastQCFastQC Report
Thu 23 Jun 2016
MU585039B_S16_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMU585039B_S16_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606634
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGC1350.002782913234.012558
AGGGGGC1853.4691795E-431.0249618
GTAGTGC1954.722505E-429.4339376
ACACTGT5655.456968E-1226.4124016
ACTGTCC4959.458745E-1025.5094138
GAGGGGG2350.001404270824.4239067
TCGTGCG3351.6464586E-523.9864649
ATAGGAC3002.15091E-422.9584733
GGGGGCT2500.00201298522.9584719
GTGTTAT4653.7059E-722.2178751
GAGTAGG3152.9922885E-421.865211
CAGGTCA3703.548825E-521.7174729
TAGAGCC3753.9359224E-521.4279089
TACACTG4855.5581404E-721.3016765
CTAGGAC2700.003147219821.2578453
ACAGTGC3253.6958785E-421.1924368
CACTGTC4355.1618426E-621.1112387
TAGGACT4405.6987556E-620.871344
TCTACAC5508.053212E-820.8713383
TACAGGG3304.0971718E-420.8713387