FastQCFastQC Report
Thu 23 Jun 2016
MG394676V_S14_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394676V_S14_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625763
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGG6700.027.4757212
CTAGGAC2956.032491E-627.3010863
CTACCCT2158.2482566E-426.756884
CCAGCGG2550.009139726.667833235
CCCTAGA2209.4287365E-426.1508581
GTATATA7100.025.9298651
GTAGTAC3151.0039652E-525.5676863
TCTAGAC4059.548239E-825.5676843
CTAGACG4201.3574936E-724.6545524
TAGGGCT2801.3266872E-424.6545524
TACCCTA2901.6832897E-423.8043985
GTCTAGG2450.001765465523.4824031
ATAGGAC3002.1180327E-423.0109163
ACTGACC3052.3688545E-422.6336868
ACTATAC4304.57892E-621.4055023
ATAAGGC5058.018742E-720.5047783
GTATACA5651.04366336E-720.3652691
ATATAAG11600.019.8385811
GTCTAGA3505.996353E-419.7252181
CTATAAC5851.5073601E-719.667453