FastQCFastQC Report
Thu 23 Jun 2016
MG394676V_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394676V_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625763
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG5003.45608E-1127.6162365
GTATAGG3405.9276863E-727.074741
ACTGTGC3006.8680856E-626.8491178
CCCTATA3108.859652E-625.9830152
GTATAGT4651.3253157E-824.745731
TACACCC2350.00138486124.482485
GTCTACA4351.9034269E-723.8070961
CTAGACT3901.959901E-623.6036194
ACTGCGC2600.002493371522.1283938
TATACTG6959.094947E-1121.5234435
TAGACTA4355.0562867E-621.1618655
CATACAC4456.1558185E-620.686323
CCAGGAC3354.462275E-420.609133
CCGAAGG3354.462275E-420.609139
CTGTGCA3404.9311883E-420.3060559
GTCCAGG3505.994994E-419.7258821
GAGAGAG2950.00518335619.502995
GCCTACA3556.5958034E-419.4480531
GTCTTAG4751.0817899E-519.3798141
GTATACT6001.9655454E-719.1779424