FastQCFastQC Report
Thu 23 Jun 2016
MG394658S_S15_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394658S_S15_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010265
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA5054.1836756E-1127.2035731
GTATAGA9750.025.831771
ACTGTGC5302.1609594E-923.7568268
GTATTAG18750.023.2016281
CTAGGAC4502.780962E-722.8929423
TACTGTG7750.022.1544597
GTCCTAT5508.300776E-820.8148561
GTGTAGG6701.4406396E-920.5041871
CCTATAT7352.0918378E-1020.2454593
CTATACA10950.019.8614564
AGAGCCC3506.214519E-419.622528
TCTATAC10400.018.7105773
ACCGTGC4901.4814483E-518.6881168
ATATAGG7404.953108E-918.56461
ACTATGC7404.962203E-918.5618448
GTATATA12500.018.3170741
CCTCTAG6253.190271E-718.3170741
GTATACG9451.6370905E-1118.17171
ATACTGT13950.018.0517836
TCCTATA8951.4915713E-1017.9050942