FastQCFastQC Report
Thu 23 Jun 2016
MG394658S_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394658S_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010265
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGC2506.375503E-527.462818
AGGCCCT1700.00872853226.9243246
TTAGGCC2400.001617800623.8392454
GAGGGGG2951.9624546E-423.273577
TGGGGTC3354.635874E-420.4946354
CCGTCCG2800.00395959320.433649
TCTATAC9800.019.8498213
CTAGGTC2900.004851241619.729034
GTACTGG8107.6761353E-1018.3650471
GTACTAC4401.3790793E-418.2045151
AGAGGGG4401.3790793E-418.2045156
TTATACT13400.017.9328064
CTTATAC12150.017.894153
GGGGGCT3200.00856358517.8794359
GTATAGA9051.7644197E-1017.7016281
GTATAAT19450.017.6496221
ACCGTAC3900.001288527417.6043668
TATACCG7207.3552656E-817.4821155
AGACCGT7359.307223E-817.1253366
CACCGTG4702.2839125E-417.0425247