FastQCFastQC Report
Thu 23 Jun 2016
MG394536Y_S20_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394536Y_S20_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588683
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCT2400.001603415923.8738214
TACACCG5452.9394869E-923.1291335
GTGTAAG5002.9909643E-822.9188671
TAGACCG2750.003535553820.8353355
CAGTGCG2750.003535553820.8353359
TATACTG7802.0008883E-1120.5682145
GCTACCC3354.587643E-420.524363
GTACTGG3955.951403E-520.3078581
ATAGCGC3405.069653E-420.222533
TCTACAC6851.869921E-920.0749233
GTATAGA6952.239176E-919.7860721
GTTATGG6552.4436304E-819.244851
TCCTAAG4251.04446895E-418.8743612
CTACACC8056.91216E-1018.5059194
CCCTTAT6202.8969225E-718.4829581
TAGACTG3800.001071305118.0938434
GTATAGG3800.001071305118.0938431
TACACTG5702.6562611E-618.0938435
CTAGTAC4451.4857989E-418.0260773
TGTAAGA8301.038643E-917.9485112